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. 2012 Jun 20;32(25):8560–8568. doi: 10.1523/JNEUROSCI.1346-12.2012

Figure 6.

Figure 6.

Membrane NKCC1 is highly glycosylated in the PVN in hypertension. a, Western blots of NKCC1 membrane proteins in the PVN from WKY rats and SHRs (top) andNKCC1 membrane protein in SHRs treated with vehicle and PNGase F (bottom). b, Quantification of the NKCC1 membrane protein level in the PVN in WKY rats and SHRs (n = 4 samples in each group). c, d, Western blots and quantification show the membrane protein levels of NKCC1 in the brainstem (c) and prefrontal cortex (d) in SHRs and WKY rats (n = 4 samples in each group). The molecular weight markers are indicated to the right of the gel. In these the protein assays, 4 samples, each consisting of PVN tissues from 3 rats, were used in each group. *p < 0.05, compared with the WKY group. Error bars represent SEM.