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. 2012 Jul 5;9:89. doi: 10.1186/1743-422X-9-89

Figure 1.

Figure 1

Identification and purification of expressed VP4.A: SDS-PAGE analysis of induced recombinant VP4 expression. M, Standard protein marker; Lane1, induced empty vector expression for 3 h as control; 2–4, cell supernatant of induced VP4 expression for 1,3,5 h respectively; 5–7, cell pellet of induced VP4 expression for 1,3,5 h respectively; A’: Western blot analysis of expressed protein samples corresponding to lane 1–7 in A with mouse anti His-tag antibody;B: SDS-PAGE analysis of purified VP4 protein. M, Standard protein marker; Lane 1, purified VP4 protein; 2, expressed VP4 protein in cell lysate pellet ; 3, empty vector control ; B’: Western blot analysis of purified VP4 protein matching Lane 1–3 in B with rabbit anti-GCRV antibody.