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Subcellular localization of the VP4 protein in transfected cells. Vero cells were transfected with pcDNA/VP4 (lower panel) or pcDNA3.1(+) (upper panel) for 24 h and then subjected to the indirect immunofluorescent assay with the VP4 antiserum, labeled with FITC-conjugated goat anti-mouse IgG, counterstained with hoechst to visualize the nuclei. The pcDNA3.1(+) was used as negative control.
