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. 2012 Jul 5;7(7):e40389. doi: 10.1371/journal.pone.0040389

Figure 4. Changes in histone modifications around SBSN TSS upon BORIS induction.

Figure 4

(A) Enrichment of active chromatin modification, H3K4me3, near the SBSN TSS was measured by ChIP experiment with H3K4me3 antibodies for H358 transfected with BORIS and induced by indicated doxycycline concentrations. The region from +202 bp to +374 bp was analyzed. Note that cell lysates from the same experiments was used for all ChIP experiments (Figure 2C). Enrichment level was measured relative to IgG in qRT-PCR as described in the Methods. (B) Enrichment of active chromatin modification – H3Ac. (C) Enrichment of repressive chromatin modification, H3K9me3. (*, p-value <0.00002 (A); p-value <0.004 (B); p-value <0.03 (C)).