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. 2012 Jul 5;7(7):e40292. doi: 10.1371/journal.pone.0040292

Figure 1. Regulation of An8547 by the UDA pathway.

Figure 1

A) 2D-PAGE images from wild-type (TN02A3) and ΔflbB (BD143) strains grown in liquid MMA medium for 18 hours. Every spot analyzed by mass spectroscopy is marked by white dotted circles and the corresponding number. Locus identification and GO term classification for proteins in each spot is shown in Table S1. B) Northern blot experiment comparing An8547 expression levels in the transition of vegetative to asexual development (18 hours of vegetative growth, labeled as 0, and 6, 12, 24 and 48 hours after the induction of conidiation) in the parental wild-type (TN02A3), ΔflbB (BD143) and ΔflbE (BD142) genetic backgrounds. RNA samples were obtained from mycelia grown using ammonium (10 mM) as the main nitrogen source. Ribosomal RNAs (rRNA) are shown as a loading control. C) Western-blot experiment comparing GmcA-HA3x levels from ΔflbB (BD142) and the parental wild type (TN02A3) vegetative mycelia (18 hours), both grown in liquid MMA containing either ammonium (10 mM; left) or nitrate (80 mM; right) as the main nitrogen source. Levels of hexokinase (Hxk) were used as a loading control.