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. 2012 Jul 5;7(7):e40292. doi: 10.1371/journal.pone.0040292

Figure 4. Phenotype characterization of ΔgmcA strain on solid and liquid media.

Figure 4

A) Colonial growth and conidiation pattern of the ΔgmcA (BD429) and ΔflbB;ΔgmcA (BD431) strains compared to their respective parentals (TN02A3 and BD177, respectively) at 48, 72, 96 and 120 hours of culture on MMA supplemented with nitrate (10 mM) and glucose (1% w/v). Scale bar  = 1.5 cm. B) Nutrient starvation induction of conidiating structures in mycelia from wild-type (row 1), ΔflbB (row 2); ΔgmcA (row 3) and the double null (ΔflbB;ΔgmcA) (row 4) strains. Mycelia were cultured for 18 hours at 37°C in MMA and subsequently transferred for additional 20 hours to standard MMA (Control; column 1), MMA without nitrogen (column 2) or MMA without carbon and ammonium (column 3) or nitrate (column 4) as nitrogen sources. FC: Fully developed Conidiophores. SC: Simplified Conidiophores. C: Single conidia emerging from a vegetative cell. Scale bar  = 50 µm.