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. 2012 Jul 5;7(7):e40292. doi: 10.1371/journal.pone.0040292

Figure 6. Effect of medium alkalinization on the conidiation capacity of null gmcA mutant.

Figure 6

A) Conidiation phenotypes after 72 hours of wild-type (TN02A3), ΔflbB (BD177), ΔgmcA (BD429) and the double null ΔflbBgmcA (BD431) strains grown on MMA supplemented with different concentrations of nitrate (upper block) or ammonium (lower block) as main nitrogen sources. Scale bar  = 2 cm. B) Growth of wild-type and mutant strains on MMA with pH values buffered to 5 (upper line), 7 (middle line) and 9 (lower line) at 96 hours of culture in MMA supplemented with urea as the main nitrogen source. Scale bar  = 1.5 cm. Graphs at the right show conidia production per square centimeter for each strain and growth condition.