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. 2012 Jul 5;7(7):e40292. doi: 10.1371/journal.pone.0040292

Figure 9. Transcriptional relationship of gmcA with the UDA pathway.

Figure 9

A) Northern blot experiments showing flbB, flbD and gmcA expression in wild type (TN02A3), ΔflbB (BD177), ΔflbD (BD198) and ΔgmcA (BD429) genetic backgrounds, during vegetative growth and asexual development. Numbers indicate the time (hours) of incubation. RNA samples were obtained from mycelia grown in liquid MMA using nitrate (80 mM) as the main nitrogen source. rRNA: Ribosomal RNAs, used as loading control. B) Phenotypes of strains overexpressing (OE) gmcA, flbB or flbD under the control of alcA promoter in wild type (BD520), ΔflbB (BD523) or ΔgmcA backgrounds (BD526; BD545 and BD543, respectively), in MMA containing 5 g.l−1 yeast extract and glucose (Glu, 1%) or threonine (Thr, 100 mM) as the carbon source and ammonium (NH4 +, 10 mM) or nitrate (NO3 −, 80 mM) as the nitrogen source. C- designates parental strains (TN02A3; BD143 and BD429, respectively) and alcAp empty strains transformed with the empty pALC-pyroA* plasmid (BD188; BD194 and BD531, respectively; [17]). Images were taken 48 hours after inoculation. Scale bar  = 2 cm.