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. 2012 Jul 5;7(7):e40315. doi: 10.1371/journal.pone.0040315

Figure 7. U2AF65/N-PU recognition displaces hnRNP H binding to the G2-G3 motifs in a dose dependent manner.

Figure 7

Supershift experiments were carried out with the G2-G3∼N-PU probe (free probes are in lanes 1 and 5), NEx from MRC5 cells (lanes 2–4, and 6–10), anti-hnRNP H antibodies (lanes 3, 7–10), antibodies against an unrelated antigen (lane 4), and increasing amounts of rU2AF65 (2, 4, and 6 µg; lanes 8–10, respectively). The complexes supershifted by the hnRNP H antibody (a-H cx) migrated back to the original position of the NEx complexes (NEx cx) as the amount of rU2AF65 increased. Probe and factors depicted as in Fig. 1C.