Table 1. Cytolytic activity of PFO derivatives with mutations in the Arg-468 residue.
Toxin | EC50 (M) | % WT |
PFO | 6.0×10−11 | 100 |
R468A | 6.0×10−9 | 1 |
R468K | 5.0×10−9 | 1 |
R468Q | 2.4×10−8 | <1 |
R468E | 3.9×10−8 | <1 |
Cytolytic activity of PFO and Arg-468 mutants is shown as the effective concentration (EC50) of toxin required for 50% lysis of human erythrocytes under standard assay conditions (see Materials and Methods for details).