Figure 5. WP1130 treatment does not inhibit proteasome activity.

RAW cells were treated with 5 µM WP1130 (WP), 50 µM MG132, 200 nM Bortezomib (Bort), or DMSO for 2 hours at 37°C. Equal amounts of protein from each cell lysate were incubated with 100 nM of the fluorogenic substrate Suc-LLVY-AMC for 60 minutes at 37°C. The fluorescence intensity for each sample was measured and normalized to the DMSO control. Data from three independent experiments with two experimental replicates per condition are presented as means +/− S.E.M. *P<0.05, ** P<0.01.