Fig 4.

Cathepsins K and V activities induced by THP-1 monocytes are significantly reduced by JNK inhibition with SP6000125. (A) HAECs were incubated with or without 10μM of SP6000125 for 1 hour, followed by conditioning with TNFα or vehicle for 4 hours. THP-1 monocytes were subsequently added, non-adhered cells were removed, and co-cultures were maintained for an additional 20 hours. Cell lysates were collected analyzed via cathepsin zymography. (B) Densitometric analysis quantified cathepsin K and cathepsin V activity (n=3, *p<0.05, SEM bars shown)