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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Bone Miner Res. 2012 Jun;27(6):1309–1321. doi: 10.1002/jbmr.1573

Fig. 4.

Fig. 4

Mutant DSPP retained in the HEK293 cell layer is reduced in size by endoglycosidase-H showing that the proteins contain rER-associated high-mannose type N-linked oligosaccharides that are often modified to enzyme-resistant forms in the Golgi network. This result suggests that the mutant proteins fail to exit the rER. SDS 4–12% NuPAGE electrophoresed in MOPS buffer, transferred to PVDF membranes and immunodetected using primary antibodies noted in Methods and the LI-COR Odyssey infrared imaging system. SeeBlue Plus2 prestained markers were used.