Fig. 3.
Enzyme-linked immunosospot assay (ELISPOT) assay [interferon (IFN)-γ] of 2-octynoic acid-bovine serum albumin (2OA-BSA)-immunized mice with natural killer (NK)1·1 antibody and with control immunoglobulin. Cytokine production of splenic mononuclear cells in response to pyruvate dehydrogenase complex (PDC)-E2 synthetic peptides using ELISPOT. The y-axis indicates the number of IFN-γ-producing T cells reactive to PDC-E2 synthetic peptides/3 × 105 splenic mononuclear cells. Note that NK1·1 antibody-treated mice had lower frequencies of autoreactive T cells than those with control immunoglobulin (P < 0·05) within 12 weeks.