Fig. 5.

Inhibition of nuclear factor (NF)-κB through the eIF2α–mammalian target of rapamycin complex 1 (mTORC1) pathway. (a,b) Cells were treated with salubrinal (a) or cordycepin (b) for the indicated time-periods, and phosphorylation of p70S6K was evaluated by Western blot analysis. Total p70S6K protein is shown at the bottom as a loading control. (c,d) NRK/NF-κB-Luc cells were stimulated with tumour necrosis factor (TNF)-α in the absence or presence of 200 nM rapamycin and 25 µM salubrinal (c) or 200 nM rapamycin and 0·5 µg/ml cordycepin (d) and subjected to luciferase assay. (e) Cells were treated with the indicated agents for 6 h and subjected to Western blot analysis of eIF2α.