Fig. 6.

Tumour necrosis factor (TNF)-α and interferon (IFN)-γ induce surface expression of transglutaminase 2 (TG2). Surface TG2 expression was evaluated by flow cytometry on non-stimulated THP-1 cells (grey line) or in cells stimulated for 20 h with TNF-α (10 ng/ml) and IFN-γ (200 UI/ml) either in the absence (black line) or the presence of sulphasalazine (10 µM) (dashed line). Cells were labelled with the 1H7H9 anti-TG2 monoclonal antibody or an isotype control (grey histogram). Mean fluorescence intensity (MFI) values are indicated for each histogram.