Table 1.
Primer sequences and probes used for TaqMan real-time polymerase chain reaction (PCR).
| Gene | Primer sequence and corresponding probe | Fragment size |
|---|---|---|
| α-ENaC | Forward: 5′-TGT GAC TAC CGA AAG CAG AGC-3′ | 102 bp |
| Reverse: 5′-AGG CTT CCG ACA CTT GGA G-3′ | ||
| Probe #26 | ||
| γ-ENaC | Forward: 5′-AGC AAC ACC CCA ACT GGA T-3′ | 93 bp |
| Reverse: 5′-AGG ATT GCT GCA CAC TGA TT-3′ | ||
| Probe #26 | ||
| α1-Na+/K+-ATPase | Forward: 5′-ACT TGG GCA CTG ACA TGG TT-3′ | 104 bp |
| Reverse: 5′-CAC AAG TTT GTC CGT TTT GG-3′ | ||
| Probe #26 | ||
| NaPi IIb | Forward: 5′-CCC AGG AAG AGG AGC AAA A-3′ | 72 bp |
| Reverse: 5′-TCA GGA GCT TTG TGC CAA C-3′ | ||
| Probe #64 | ||
| Aquaporin 5 | Forward: 5′-GCT CCG AGC TGT CTT CTA CG-3′ | 131 bp |
| Reverse: 5′-GCG TTG TGT TGT TGT TCA GC-3′ | ||
| Probe #20 | ||
| 18S | Forward: 5′-GGA GAG GGA GCC TGA GAA AC-3′ | 70 bp |
| Reverse: 5′-TCG GGA GTG GGT AAT TTG C-3′ | ||
| Probe #74 | ||
| Probe #20 | 5′-CTG GCT GG-3′ | |
| Probe #26 | 5′-CAG CCC AG-3′ | |
| Probe #64 | 5′-CCA GGC TG-3′ | |
| Probe #74 | 5′-GGC AGC AG-3′ |
α-ENaC: alpha-subunit of the epithelial amiloride-sensitive Na+-channel; γ-ENaC: gamma-subunit of the epithelial sodium channel; α1-Na+/K+-ATPase: sodium-potassium-adenosine-triphosphatase (α1-subunit); AQP5: Aquaporin 5; NaPi IIb: sodium-dependent phosphate co-transporter type IIb; 18S: housekeeping gene (ribosomal origin); bp: base pairs.