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. 2012 Mar 30;220(6):603–611. doi: 10.1111/j.1469-7580.2012.01503.x

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© 2012 The Authors. Journal of Anatomy © 2012 Anatomical Society

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Fig. 3 — Quantification of muscle collagens and MMP-9 in myostatin null muscle. (a) Western blots of collagen I, III, IV and MMP-9 expression in BB muscle of 4-month-old and 19-month-old mstn^+/+ and mstn^−/− mice. Collagen I, III, IV and MMP-9 were recognised as bands of 160 kDa, 250 kDa, 130 kDa, 120 kDa, respectively. Tubulin was used as an internal loading control. (b, c) Quantification of collagen I and III Western blot bands from BB muscle of 4-month-old and 19-month-old mstn^+/+ (red) and mstn^−/− (green) mice. Two-way anova revealed significant reduction in the amount of the collagen I and III bands in 4-month-old mstn^−/− compared with age-matched mstn^+/+ (P = 0.001 and P < 0.001), respectively. A significant increase in protein levels in 19-month-old mstn^+/+ compared with 4-month-old mstn^+/+ was detected for collagen I and III (P < 0.05 and P = 0.08), respectively. Significant interaction (age vs. genotype) indicates the difference between both genotypes with ageing for collagen I and III (P < 0.05 and P = 0.001), respectively. (d) Quantification of the band of collagen IV showed a significant reduction in the amount of collagen IV (P < 0.001) in 4-month-old mstn^−/− compared with age-matched mstn^+/+. Two-way anova demonstrated a significant increase in collagen IV band of 19-month-old mstn^+/+ compared with age-matched mstn^+/+ (P < 0.05). No significant difference was observed in 19-month-old mstn^−/− compared with 4-month-old mstn^−/−. (e) Quantification of Western blot bands showed a significant reduction in the amount of MMP-9 (P < 0.001) in 4-month-old mstn^−/− (green) compared with age-matched mstn^+/+ (red). A significant increase in the MMP-9 levels was found in 19-month-old mstn^+/+ compared with 4-month-old mstn^+/+ (P < 0.001). A significant interaction (genotype vs. age) was found between both genotypes with ageing (P < 0.001). Band intensities were plotted relative to tubulin. All data presented as mean ± SEM.