Fig. 3.

Double-label confocal microscopy in adult mouse hypothalamus. CNTF-positive tanycyte-like cells (A and D, arrowheads) also express vimentin (B and C, arrowheads) and GFAP (E and F, arrowheads). Note one CNTF-positive tanycyte-like cell (A, arrow) that is not positive for vimentin; in turn, some tanycytes exhibiting vimentin IR (B, arrows) are not positive for CNTF. CNTF-positive astrocyte-like cells (G, arrows) are also positive for GFAP (H and I, arrows). In (A), CNTF was detected with the Promega antibody. 3V, third ventricle; CNTF, ciliary neurotrophic factor; GFAP, glial fibrillary acidic protein. Scale bar: 40 μm.