Fig. 7.

Local inhibition of soluble tumour necrosis factor (sTNF) and transmembrane (tm)TNF is required to suppress experimental autoimmune uveoretinitis (EAU). EAU was induced in B10.RIII mice, and on day 9 post-immunization (p.i.) mice were treated intravitreally with 10 µg/eye of human immunoglobulin (Ig)G or sTNFR-Ig (same mouse, contralateral eyes) or 10 µg/ml of I-XPro or XPro1595. Clinical progression of disease was assessed by topical endoscopic fundus imaging (TEFI) from days 8 to 15 p.i. (a,b). The P-value for each day is stated in the table beneath the graphs. On day 15, the mice were killed and eyes taken for histological analysis (c,d); mean ± standard error shown, n = 9–11 mice per group. The Mann–Whitney U-test was used for statistical analysis. Histological sections were used to assess F4/80⁺ (a mouse macrophage marker) (e) and CD4⁺ (f) cell infiltrate. Histological sections were also used to assess nitrotyrosine expression (red) in conjunction with 4′,6-diamidino-2-phenylindole (DAPI) (blue); ×20 magnification (g).