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. 2012 May 24;3:187. doi: 10.3389/fmicb.2012.00187

FIGURE 1.

FIGURE 1

Cryptococcus neoformans cells lacking the ISC1 gene are sensitive to HU and MMS. (A) Ten-fold serial dilutions of log-phase cultures of C. neoformans (WT, isc1Δ, and isc1Δ + Cn ISC1 reconstituted) and S. cerevisiae (WT and isc1Δ strains) were spotted on plates containing YPD and YPD + HU (50 mM). (B) Ten-fold serial dilutions of log-phase cultures of C. neoformans (WT, isc1Δ, and isc1Δ + Cn ISC1 reconstituted) were spotted on YPD and YPD + 0.033% MMS plates. (C) Cn ISC1 complements HU sensitivity of the S. cerevisiae isc1Δ strain. Ten-fold serial dilutions of following log-phase cultures were spotted on SD/Ura- and SD/Ura-/HU plates: Sc WT cells containing pYES vector, Sc isc1Δ cells containing the pYES vector, Sc isc1Δ cells containing pYES-Sc ISC1 and Sc isc1Δ cells containing pYES-Cn ISC1.