FIGURE 3.

Synergistic role of HU and ISC1 deletion on Cryptococcus infection of the macrophage-like cell line J774A.1. (A) Phagocytosis assay with macrophage-like cell line J774A.1 cells infected with Cn WT or isc1Δ cells in the presence/absence of HU (see Materials and Methods). (B) Cn cells phagocytosed by J774A.1 were grown in the presence/absence of HU and stained with Giemsa after 24 h. Photographs were taken with a Zeiss microscope equipped with a CCD camera. All images were captured under oil immersion (×100). Red arrows indicate the presence/absence of Cryptococcus in the phagolysosomes of macrophages. Most phagolysosomes in untreated WT cells contained Cn cells, whereas some HU-treated WT cells still contained Cn cells. In contrast, most macrophages did not contain Cn isc1Δ cells regardless of HU treatment.