Table 2. Summary of Challenge Data.
| Group | Number of Mice | Day of Scheduled Euthanasia (post-challenge) | Target challenge dosage (cfu/mouse) | Calculated inhaled dosage (cfu) |
|---|---|---|---|---|
| Aerosola | ||||
| Low Dose | ||||
| Aerosol | ||||
| Challenge Study | ||||
| 1 | 20 | Day 54 | 3.00E+01 | 2.30E+01 |
| 2 | 20 | Day 54 | 1.00E+02 | 7.40E+01 |
| 3 | 20 | Day 55 | 3.00E+02 | 2.63E+02 |
| 4 | 20 | Day 56 | 1.00E+03 | 9.48E+02 |
| 5 | 20 | Day 56 | 0.00E+00 | 0.00E+00 |
| Kinetics of | ||||
| Pathophysiology | ||||
| Study | ||||
| 6 | 11 | Day 14 | 1.00E+04 | 2.26E+04 |
| 7 | 11 | Day 28 | 1.00E+04 | 2.26E+04 |
| 8 | 11 | Day 42 | 1.00E+04 | 2.28E+04 |
| 9 | 12 | Day 56 | 1.00E+04 | 2.28E+04 |
| 10 | 12 | Day 14 | 1.00E+05 | 2.15E+05 |
| 11 | 12 | Day 28 | 1.00E+05 | 2.15E+05 |
| 12 | 11 | Day 42 | 1.00E+05 | 2.38E+05 |
| 13 | 11 | Day 56 | 1.00E+05 | 2.38E+05 |
| 14 | 12 | Day 14 | 5.00E+05 | 8.73E+05 |
| 15 | 11 | Day 28 | 5.00E+05 | 8.73E+05 |
| 16 | 12 | Day 42 | 5.00E+05 | 1.12E+06 |
| 17 | 12 | Day 56 | 5.00E+05 | 1.12E+06 |
| 18 | 12 | Day 56 | 0.00E+00 | 0.00E+00 |
| Intranasal | ||||
| 19 | 12 | Day 14 | 1.00E+04 | |
| 20 | 12 | Day 21 | 1.00E+04 | |
| 21 | 12 | Day 42 | 1.00E+04 | |
| 22 | 12 | Day 56 | 1.00E+04 |
aCalculated based on estimated concentration of B. melitensis 16 M in the exposure chamber and the total volume of air inhaled by the mice.
The aerosol concentration (cfu/L) was calculated as follows: AC = (C × V)/(S × T), where AC = Aerosol Concentration (cfu/L), C = AGI concentration (cfu/mL), V = AGI sampler volume (mL), S = sampling rate (∼6 L/min), and T = Exposure time (min).