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Structure-based sequence alignment (50) of Ac-, Ls-, and BtAChBPs with the α4, β2, and α7 nAChR subunits. Ligand binding site residues involved in nicotine binding are boxed. Principal face residues are colored and boxed in blue, whereas complementary face ligand binding residues are colored and boxed in orange. Residues that were mutated to reflect α4β2 and α7 nAChRs are indicated by a vertical green arrow.
