FIGURE 6.

A, growth curves of E. coli DH5α transformed with plasmids that encode wild type Pst fused to the MalE signal sequence at 30 °C. Transcription was induced with 0.1% arabinose (■) and 0.001% arabinose (▾) or repressed by 0.2% glucose (glc) (●). Cells transformed with the mutated MalE′-Pst^E178A plasmid were not inhibited after induction with 0.1% arabinose (□). B, growth curves of E. coli SIP1332 fyuA⁺ transformed with plasmids that encode Pst^A fused to the MalE signal sequence at 37 °C. Transcription was induced with 0.1% arabinose (■), 0.01% arabinose (▴), or repressed by 0.2% glucose (●). Cells transformed with the mutated MalE′-Pst^A(E178A) plasmid were not lysed after induction with 0.1% arabinose (□) and 0.01% arabinose (Δ).