TABLE 3.

Binding of Pst^RT, Pst^D207A, and Pst^Δ10–20 proteins to the FyuA receptor determined by competition with Pst-T4L

Pst^A was used as a negative control. The numbers indicate the diameters of lysis zones (in mm) that were formed in a plaque assay on LB agar plates seeded with E. coli SIP1332 (fyuA⁺) onto which 6 μl of the protein mixture with the molar ratio indicated was applied. The concentration of Pst-T4L was 10 μm. – indicates no lysis zone; turbid lysis zones are given in parentheses, very turbid lysis zones in double parentheses; ND means not determined.

Protein or ratio Pst-T4L:Pst	PstRT	PstD207A	PstΔ10–20	PstA
Pst-T4L, no Pst derivative	11	11	11	11
1:2	(8)	(7.5)	ND	11
1:5	((8))	((7.5))	–	11
1:10	–	–	–	11
1:20	–	–	–	11
1:50	–	–	–	11