TABLE 5.
In vivo activities of oxidized and reduced Pst-T4L and Pst disulfide derivatives
The numbers indicate the diameters of lysis zones (turbid lysis zones in parentheses) that were formed on LB agar plates seeded with the pesticin-sensitive E. coli strain SIP1322 fyuA+ onto which 6 μl of serial 101- to 105-fold dilutions of pesticin solutions were placed. The concentration of the stock solution was 10 μm for Pst-T4L wild type and derivatives and 2 mm for Pst wild type and derivatives. – indicates no lysis zone.
| Protein | Dilution |
||||
|---|---|---|---|---|---|
| 1:101 | 1:102 | 1:103 | 1:104 | 1:105 | |
| Pst-T4L wild type | 11 | 9 | 6.5 | (5) | – |
| Pst-T4L wild type + DTT | 11 | 9 | 6.5 | (5) | – |
| Pst-T4L wild type + Cu2+ | 11 | 9 | 6 | (5) | – |
| Pst-T4LI176C/L331C | – | – | – | – | – |
| Pst-T4LI176C/L331C + DTT | 11 | 9.5 | 7.5 | (5) | – |
| Pst-T4LS257C/Q289C | – | – | – | – | – |
| Pst-T4LS257C/Q289C + DTT | 10 | 8 | (8) | – | – |
| Pst-T4LD294C/R321C | 10 | 8 | 6 | (5) | – |
| Pst-T4LD294C/R321C + DTT | 10 | 8 | 6 | (5) | – |
| Pst-T4LD294C/R321C + Cu2+ | (10) | – | – | – | – |
| Pst-T4LI176C/R321C | 9 | (7.5) | – | – | – |
| Pst-T4LI176C/R321C + DTT | 9 | 7.5 | (6) | – | – |
| Pst wild type | 16 | 14.5 | 12 | 10 | 7 |
| PstS89C/S285C | – | – | – | – | – |
| PstS89C/S285C + DTT | 16.5 | 15 | 13 | 10.5 | 8 |