FIGURE 6.

D609 and HDL reduce the specific radioactivity of TG in primary mouse hepatocytes labeled with [³H]oleic acid. Mouse primary hepatocytes were incubated for 1 h with or without the PC-PLC inhibitor D609 after which the cells were incubated for 4 h with [³H]oleic acid in the presence or absence of various concentrations of human HDL (final concentration of PC, 0, 1, or 2 mm). The concentrations of PC (A) and TG (B) as well as the radioactivity in these lipids were measured. C and D show the specific activity of PC and TG, respectively, relative to that in control cells incubated without HDL and/or D609. Values are means ± S.E. (error bars) of three independent hepatocyte preparations. *, significant difference versus no HDL with the same D609 treatment; #, significant difference versus no D609 treatment with the same HDL concentration determined using one-way analysis of variance tests (p < 0.05).