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The measurable Atf1 activity in Δsty1Δpmk1 cells is scavenged by NAC. Wild-type, Δsty1, and Δsty1Δpmk1 cells transformed with the Renilla reporter plasmid were treated with or without NAC (0.1 or 0.5 mm) before CdCl2 (2 mm) and H2O2 (2 mm) were added as described under “Experimental Procedures.” The reporter activities in cells were compared with the activity in the presence of 0.5 mm NAC alone.
