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HIRA complex mutants are especially sensitive to low-dose stress. Ten-fold serial dilutions of wild-type (JK317), slm9Δ (MC3749), hip1Δ (MC3725), hip3Δ (MC3773), hip4Δ (MC3799), and spc1Δ (MC3801) cells were spotted onto YES plates or YES plates containing H2O2, menadione, KCl, or sorbitol. For heat stress, the spotted YES plates were subjected to the indicated heat stress dose.
