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. 2012 May 15;287(28):23440–23450. doi: 10.1074/jbc.M112.349944

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — HIRA is recruited to stress-responsive gene loci upon stress treatment. A, slm9-12myc (MC3793) and hip1-12myc (MC3795) cells were exposed to 40 °C for the indicated times. Whole cell extracts were prepared and analyzed by Western blotting with anti-Myc and anti-Cdc2 (control) antibodies. B, slm9-12myc (MC3793) and slm9-12myc atf1Δ (MC4219) cells were exposed or not exposed to 40 °C for 15 min. ChIP assay using anti-Myc antibody was performed. Purified DNA was analyzed by real-time PCR using primer sets for the promoter (prom) and coding (ORF) regions of stress-responsive genes (ctt1⁺, gpx1⁺, and hsp9⁺), non-stress-responsive gene (pol1⁺), and heterochromatic locus (outer repeats of centromere, dh). Values shown were normalized to cdc2⁺ promoter. Results are the means of three independent experiments, and error bars represent S.E..