FIGURE 5.

HIRA is required for stress-responsive gene expression and Pol II recruitment. A, wild-type (JK317), slm9Δ (MC3749), and hip1Δ (MC3725) cells were exposed or not exposed to 40 °C for 1 h. Total RNA was analyzed by RT-PCR using primer sets for stress-responsive genes (ctt1⁺, gpx1⁺, gpd1⁺, tps1⁺, hsp9⁺, and hsp16⁺). act1⁺ is shown as the loading control. B, wild-type (JK317) and slm9Δ (MC3749) cells were exposed or not exposed to 40 °C for 15 min. ChIP assay using anti-Pol II antibodies was performed. Purified DNA was analyzed in the same way as described in Fig. 4B. Results are the means of three independent experiments, and error bars represent S.E.