FIGURE 7.

HIRA is required for nucleosome eviction at stress-responsive gene locus. Wild-type (JK317) and slm9Δ (MC3749) cells were exposed or not exposed to 40 °C for 15 min. Mononucleosomal DNA was isolated from the cells, and nucleosome-scanning analysis was performed. Real-time PCR was carried out using 10 overlapping primer sets along ctt1⁺ gene. Nucleosomal DNA enrichment is defined by the ratio of the amplified products with mononucleosomal DNA to genomic DNA. Results are the means of three independent experiments, and error bars represent S.E. Inferred locations of nucleosomes (light gray ovals) with respect to ORF (white rectangle) and TSS (black arrow) are shown. TATA box (−30 to −23) and Atf1 binding site (−57 to −50) are also represented as black rectangle and dark gray rectangle, respectively (55).