TABLE 1.
Cytokine release in response to LDL and different forms of copper-oxidized LDL in human monocytes
Human monocytes (2 × 106/ml) were treated with native LDL and copper-oxidized LDL for 3 and 6 h. The cell supernatants were measured for IL-6 and IL-8 release by ELISA. Values are represented as mean ± S.E. for three independent experiments.
| Samples | IL-6 (ng/ml) |
IL-8 (ng/ml)) |
||
|---|---|---|---|---|
| 3 h | 6 h | 3 h | 6 h | |
| Control | 0.05 ± 0.03 | 0.06 ± 0.04 | 0.41 ± 0.08 | 1.04 ± 0.41 |
| Native LDL | 0.04 ± 0.03 | 0.11 ± 0.05 | 0.44 ± 0.07 | 0.86 ± 0.39 |
| Native LDL + Cu2+ (5 μm) | 0.02 ± 0.01 | 0.05 ± 0.03 | 0.48 ± 0.04 | 0.85 ± 0.37 |
| mmLDL | 0.03 ± 0.01 | 0.06 ± 0.03 | 0.47 ± 0.05 | 1.11 ± 0.40 |
| oxLDL | 0.02 ± 0.00 | 0.12 ± 0.03 | 0.47 ± 0.021 | 1.35 ± 0.39 |
| oxmLDL | 0.00 ± 0.00 | 0.01 ± 0.01 | 0.75 ± 0.34 | 1.84 ± 0.23 |
| LPS (100 ng/ml) | 3.4 ± 0.25 | 25 ± 7 | 5.7 ± 1.1 | 16 ± 1.5 |
| LPS (10 ng/ml) | 3.7 ± 0.53 | 25 ± 10 | 4.9 ± 0.9 | 16 ± 1.6 |