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. 2012 May 2;287(28):23489–23501. doi: 10.1074/jbc.M112.365874

FIGURE 4.

FIGURE 4.

SIRT1 activation by cAMP/PKA/AMPK pathway depends on DBC1. A, SIRT1 activity was measured in WT and DBC1 KO MEFs. Cells were incubated with A769662 (100 μm) or A769662 + compound C (CC), AICAR (2 mm) or AICAR + compound C, and oligomycin (5 μm) or oligomycin + compound C for 2 h before measuring SIRT1 activity. Compound C was used at 10 μm and was preincubated for 2 h. Activity in the WT cells was normalized to the WT control, and activity in the KO cells was normalized to the KO control. SIRT1 activity in the control was always higher in DBC1 KO than in WT MEFs (see Fig. 1). *, p < 0.05 (ANOVA test, n = 3). B, DBC1 was knocked down in HepG2 cells with siRNA, and SIRT1 activity was assessed after stimulation with 10 μm forskolin (FSK) for 10 min. Activity is shown as -fold change with respect to the control. *, p < 0.05 (ANOVA test, n = 3). C, SIRT1 activity in DBC1 WT and KO MEFS treated with 10 μm forskolin for the indicated times. *, p < 0.05 (ANOVA test, n = 3). Error bars represent S.D.