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bLF inhibits the IL-1β and flagellin-mediated NFκB pathway. ST2 cells were pretreated with the indicated concentration of bLF or vehicle before IL-1β (10 ng/ml) (A) or flagellin (100 ng/ml) (B) stimulation, respectively. After each stimulation cells were collected for examining the expression of TNF-α and for immunoblotting with antibodies against p-IKKβ, IKKβ, IκBα, and β-actin. The experiments were performed at least twice, with similar results. **, p < 0.01.
