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. 2012 May 23;287(28):23537–23548. doi: 10.1074/jbc.M112.363960

FIGURE 4.

FIGURE 4.

Mitochondrial oxygen consumption is increased and dysregulated lipogenesis in DIO mice is corrected by MSDC-0602 treatment. A, mitochondrial respiration was performed on isolated mitochondria and using succinate-rotenone as a substrate. *, p < 0.05 versus other groups mice. FCCP, carbonylcyanide-p-trifluoromethoxyphenylhydrazone. B, the graph depicts the hepatic expression of genes encoding glycolytic and lipogenic enzymes. n = 8 per group. *p < 0.05 versus lean mice. **p < 0.05 versus DIO and lean mice. Inset, representative Western blots using liver lysates and the antibodies listed at the left are shown. The table represents the normalized and corrected (to actin) band intensity for FAS (n = 6 per group). AU, absorbance units. C, the graph depicts rates of fatty acid synthesis in hepatocytes. *, p < 0.05 versus lean mice. **, p < 0.05 versus DIO and lean mice. D, the graph depicts the hepatic expression of genes encoding enzymes involved in TG esterification. *, p < 0.05 versus lean mice. **, p < 0.05 versus DIO and lean mice. E, the graph depicts rates of triglyceride synthesis in hepatocytes. *, p < 0.05 versus lean mice. **, p < 0.05 versus DIO and lean mice.