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. 2012 May 21;287(28):23690–23697. doi: 10.1074/jbc.M112.366955

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Multiple HCN channel subtypes selectively associate with β₂AR but not with other ARs. A, protein complex formation between the HCN4 channel and β₂AR. The indicated combinations of V5 epitope-tagged HCN4 and FLAG-tagged ARs were transiently expressed in HEK cells and subjected to immunoprecipitation (IP) using anti-FLAG antibody. Upper panel, β₂AR selectively coprecipitated the HCN4 channel. Middle panel, immunoprecipitated FLAG-tagged ARs. Lower panel, summary of the quantification of three independent experiments. B, upper panel, β₂AR coprecipitated HCN1-V5. Lower panel, summary of the quantification of three independent experiments. C, upper panel, β₂AR coprecipitated HCN2-GFP. Lower panel, summary of the quantification of three independent experiments. Error bars show S.E.