FIGURE 3.

Synthetic peptide HBAR disrupts the HCN channel-β₂AR interaction. A, pretreatment with HBAR but not shHBAR reduced co-immunoprecipitation of the HCN4 channel. HCN4-V5 and FLAG-β₂AR were coexpressed in HEK cells and subjected to immunoprecipitation (IP) using anti-FLAG antibody. B, pretreatment with HBAR did not alter HCN4 protein amount at the plasma membrane (surface HCN4) and in the whole cell lysate. C, pretreatment with HBAR reduced β₂AR-bound HCN1. D, pretreatment with HBAR reduced β₂AR-bound HCN2. Error bars show S.E.