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HBAR eliminates adrenergic facilitation of spontaneous beating of SAN cells. A, spontaneous contraction of SAN cells was monitored by live cell imaging. Optical signals from the edges of cells were measured to detect cell movements. Spontaneous beating of shHBAR-treated cells was accelerated by ISO. B, HBAR-treated SAN cells did not respond to ISO. C, summary of the adrenergic effect on spontaneous beating. **, < 0.01. Error bars show S.E.
