Voltage-gated calcium currents from ventricular cardiomyocyte are not affected by HBAR peptides.
A, current traces of voltage-gated calcium currents before (upper left) and after (upper right) application of ISO. Cells were clamped at a holding potential of −80 mV and depolarized to potentials between −80 and 50 mV in steps of 10 mV for 500 ms. Lower, voltage-current relationship obtained from the traces shown. B, time course for adrenergic modulation of calcium currents from untreated (open circles), HBAR-treated (blue circles), and shHBAR-treated (red triangles) myocytes are shown. Inset, current traces at t = 0 and t = 1 min evoked by 100-ms step depolarizations to −10 mV from a holding potential of −80 mV. C, summary of adrenergic modulation of calcium current. A histogram of currents at t = 1 min in B is shown for comparison. Error bars show S.E.