FIGURE 2.
Intermolecular NOEs in the IIAChb*(H89E)-HPr complex. NOEs in a three-dimensional 12C-filtered/13C-separated NOE experiment recorded in D2O are specifically observed from protons attached to 12C (in the F1 dimension) to methyl protons attached to 13C (in the F3 dimension). Strips are shown for NOEs involving the 13Cδ1 methyl group of Ile-72 (at 12.48 ppm) and one of the 13Cγ methyl groups of Val-21 (at 23.63 ppm) of IIAChb*(H89E). The amino acid (AA) specific labeling schemes used for [1H-AA]/[2H,12C,14N]HPr are shown above each strip.