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Representative pre-steady state burst kinetic plots of wild-type pol β (A) and G139E (B). A 1-bp gapped DNA substrate was incubated with pol β and dNTP on a millisecond or minute time scale as described under “Experimental Procedures.” Extended product formed was plotted as a function of time and fit to the biphasic burst equation for wild type with a kobs of 14 ± 3/s. Extended product formed by G139E was fit to a single exponential equation with a kobs of 0.004 ± 0.0001/s.
