TABLE 2.

Algorithm predictions and gap-filling activity of pol β variants

Damaging variant	SIFT	PolyPhen	In vitro enzyme activity relative to WTa
N24K	Tolerated	Possibly damaging	Normal
S44F	Tolerated	Probably damaging	Normal
E58G	Affect function	Probably damaging	Normal
P63L	Affect function	Benign	Normal
T79I	Affect function	Probably damaging	Normal
G80V	Affect function	Probably damaging	Greatly reduced
T101I	Tolerated	Possibly damaging	Normal
R112W	Tolerated	Probably damaging	Normal
T121I	Affect function	Probably damaging	Reduced
H134Y	Affect function	Probably damaging	Normal
H135Y	Tolerated	Probably damaging	Normal
G139E	Affect function	Probably damaging	Greatly reduced
T176I	Tolerated	Possibly damaging	Normal
P198L	Affect function	Probably damaging	Normal
S229L	Tolerated	Possibly damaging	Reduced
G231D	Tolerated	Probably damaging	Greatly reduced
L241F	Affect function	Probably damaging	Normal
P242L	Affect function	Probably damaging	Normal
S275N/E295K	Affect function	Probably damaging	Undetectable
T292I	Tolerated	Probably damaging	Greatly reduced
E295K	Affect function	Probably damaging	Undetectableb

^a Data are as measured by the 5-bp gap filling assay.

^b Variant was previously identified in gastric tumor (33) and characterized (25).