TABLE 2.

Single turnover misincorporation opposite template A

Kinetic constants obtained from single turnover misincorporation experiments are listed for each enzyme (± S.E. of the fit).

dNTP	Enzyme	kpol	Kd	kpolc/kpolia	Kdi/Kdca	Efficiencyb	Fold changec
		s−1	μm			μm−1 s−1
dTTP	WT	46 ± 2	35 ± 5			1.3	55
	G231D	13.1 ± 0.9	550 ± 70			2.4 × 10−2
dATP	WT	0.025 ± 0.001	57 ± 10	1800	1.6	4.4 × 10−4	58
	G231D	0.00250 ± 0.00007	330 ± 30	5200	0.60	7.6 × 10−6
dCTP	WT	0.079 ± 0.004	280 ± 40	580	8.0	2.8 × 10−4	110
	G231D	0.0016 ± 0.0002	610 ± 110	8200	1.1	2.6 × 10−6
dGTP	WT	0.031 ± 0.002	84 ± 18	1500	2.4	3.7 × 10−4	93
	G231D	0.00119 ± 0.00007	300 ± 50	11,000	0.55	4.0 × 10−6

^a Subscript c denotes correct nucleotide; subscript i denotes incorrect nucleotide.

^b Efficiency was calculated by dividing k_pol by K_d for each condition.

^c Fold change in efficiency (WT/G231D) is shown.