FIGURE 2.

Overview of the Pla-Alp11 structure. A, side view of the extracellular part of the complex shown schematically. Extracellular loops are labeled (L1–L5). Alp11 residues (Lys⁵⁵⁷-Lys⁵⁶⁷) are shown as pink stick models. The Cys⁵⁵⁸-Cys⁵⁶⁶ disulfide bond is colored yellow. The active site residues (Asn⁸⁴, Asp⁸⁶, Asp²⁰⁶, and His²⁰⁸) are shown in yellow; the other residues that are important for activity are shown in cyan (Ser⁹⁹ and His¹⁰¹) and green (Arg²¹¹). Residues in the two acidic pockets are shown in gray (Asp⁹⁷, Asn¹⁵¹, Glu²⁹, Asp²⁰⁴, and Glu²¹⁷). B, stereoview of the Pla-Alp11 active site from the side, 90° rotated relative to A. Residues of the peptide are labeled in italics. 2 F_o − F_c map density is shown as a green mesh, contoured at 0.7 σ.