TABLE 2.

Kinetic parameters of the interaction of soluble CD95L and cellular CD95

Cell line	KD of GpL-FLAG-CD95La	Binding sites per cell	KD of FLAG-CD95Lb	Koffc	Kond	KD = Koff/Kon
	pm		pm	s−1	m−1s−1	pm
HT1080	2000 ± 720	23000 ± 7900	840 ± 90	1.0 × 10−3	2.9 × 105	3320
HaCaT	2600 ± 1500	4600 ± 2600	850 ± 120	1.5 × 10−3	1.7 × 105	8740

^a Derived from equilibrium binding study. n = 3.

^b Derived from homologous competition assay. n = 3.

^c n = 3–4 experiments.

^d n = 3–4 experiments.