FIGURE 3.

Hypoxic stasis results in the progressive degradation of the ribosome that is manifested by an imbalance in the 30S/50S ratio. The abundance of ribosomal peptides (confidence score of at least 95% in ProteinPilot) from each biological sample was compared with the corresponding abundance in control samples (actively growing M. smegmatis). The logarithms of the ratios of peptides corresponding to either 30S or 50S ribosomal subunits were pooled, and their distribution was plotted (data were split into 25 bins). A, comparison of ribosomal peptides from actively growing cells. B, carbon-starved ribosomes versus exponential phase ribosomes. C, hypoxic stasis of the indicated strain and time versus exponential phase for 30S ribosome peptides. D, hypoxic stasis of the indicated strain and time versus exponential phase for 50S ribosomal peptides.