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. 2012 Apr 27;287(28):24053–24063. doi: 10.1074/jbc.M112.364851

FIGURE 4.

FIGURE 4.

Loss of MSMEG_3935 is the major factor leading to the ΔdosR mutant phenotype. A, genetic context of MSMEG_3935 and Rv0079. PEP, synthase-phosphoenolpyruvate synthase; TF, transcription factor; USP, universal stress protein. The numbers indicate the standard annotation of genes whose function is unknown. The figure is not drawn to scale. MSMEG_3935 has a putative DosR-responsive element upstream of its start codon. The DosR response of element of MSMEG_3935 has a high degree of sequence identity of that of Rv0079 (underlined). Letters in bold are in agreement with the consensus determined by Park et al. (9). B, viability of the mc2155 wild type, ΔdosR, and ΔMSMEG_3935 mutants and the complemented strains ΔdosR(dosR), ΔdosR(MSMEG_3935), andΔMSMEG_3935(MSMEG_3935) during hypoxic stationary phase was determined. Triplicate cultures were grown in LB media + 0.05% Tween 80, and viability was determined by plating at the times indicated. Error bars represent mean ± S.D. CFU, colony-forming units. C, viability of the mc2155 wild type, ΔdosR, and ΔMSMEG_3935 mutants and the complemented strains ΔdosR/DosRc, ΔdosR/MSMEG_3935c, andΔMSMEG_3935/MSMEG_3935c during 55 °C heat shock was determined. Triplicate cultures were grown at 37 °C in LB media + 0.05% Tween 80 into hypoxic stationary phase. After 1 day in stationary phase, cultures were transferred to 55 °C, and viability was determined by plating at the times indicated. Error bars represent mean ± S.D.