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. 2012 Apr 27;287(28):24053–24063. doi: 10.1074/jbc.M112.364851

FIGURE 5.

FIGURE 5.

MS analysis using multiple reaction monitoring indicates that MSMEG_3935 is absent from hypoxic M. smegmatis ΔdosR cultures. MSMEG_3935 is absent from M. smegmatis ΔdosR ribosomes during hypoxia. Ribosomes from hypoxic stationary phase cultures of M. smegmatis wild type (A) and M. smegmatis ΔdosR (B) were isolated and analyzed by tandem mass spectrometry using the multiple reaction monitoring method. The transitions used to detect peptides from MSMEG_3935, MSMEG_1878, and ribosomal proteins L7/12 and S2 are listed in supplemental Table S4.